![]() With our protocol, cell indexing can be performed in the Fluidigm C1 microfluidic device, resulting in a significant reduction of cost and labor. We developed an optimized 5΄ selective workflow where all the cell indexing is done prior to fragmentation. The only currently existing 5΄ selective approach requires costly and labor intensive fragmentation and cell barcoding after cDNA amplification. ![]() 5΄ selective transcriptome profiling approaches allow simultaneous definition of the transcription start size and have advantages over 3΄ selective approaches which just provide internal sequences close to the 3΄ end. Single cell RNA sequencing approaches are instrumental in studies of cell-to-cell variability. ![]() Free download program buku pemasaran philip kotler edisi 13 reasons.
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